Practical and Research

What is SDS PAGE? The full form of SDS PAGE is sodium dodecyl sulphate – polyacrylamide gel electrophoresis. It was developed by biochemistry professor Ulrich K. Laemmli. It is a powerful, well-established and quite an elaborate electrophoresis technique used to obtain high-resolution analytical separation of protein aggregates and fragments. SDS PAGE is an easy, advanced, … [Read more...] about SDS PAGE Protocol

There are several assays for the determination of cell viability and cytotoxicity. Some of the assays that use drug exposure, irradiation etc. do not manifest the immediate result; instead, it may take several hours or sometimes even days to acquire results. In such a scenario, MTT, which is a survival assay that delivers instant results based on retention of regenerative … [Read more...] about MTT Assay

In analytical and clinical research, chromatography techniques are the gold standards for the separation of components in any given sample. The basic chromatography principle is the segregation of the constituent components present in the sample based on their interaction with two phases, i.e., the inner matrix (stationary phase) and the mobile phase. Ion exchange … [Read more...] about Ion Exchange Chromatography

You might have studied about gas chromatography and mass spectrometry techniques. The former is for the separation, whereas the latter works for the detection and identification of the components of the desired sample. Gas chromatography-mass spectrometry is a combination of the two techniques. It facilitates the separation as well as identification of the constituents … [Read more...] about Gas Chromatography Mass Spectrometry (GCMS)

Just like humans, microorganisms also require food for survival and growth. Naturally growing microbes get the necessary nutrients from their surrounding environment. But when we culture them in a laboratory in order to identify the infectious agent or for research, quality control, or serological testing, we need to provide them with an artificial food source. This food is … [Read more...] about Nutrient Agar Media